RUFY1 Rekombinanter Antikörper

RUFY1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250159A3

Kat-Nr. : 85839-4-RR

Synonyme

FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, RABIP4, RUFY 1

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  WB analysis using 85839-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85839-4-RR (RUFY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 85839-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RUFY1 antibody (85839-4-RR, Clone: 250159A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85839-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85839-4-RR against Human RUFY1 were performed. The affinity constant is 0.444 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85839-4-RR bindet in WB, IF/ICC, ELISA RUFY1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RUFY1 fusion protein Ag4324
• Vollständiger Name: RUN and FYVE domain containing 1
• Berechnetes Molekulargewicht: 708 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 69 kDa
• GenBank-Zugangsnummer: BC032571
• Gene symbol: RUFY1
• Gene ID (NCBI): 80230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.