RUFY3 Rekombinanter Antikörper

RUFY3 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240519E7

Kat-Nr. : 83601-2-PBS

Synonyme

240519E7, KIAA0871, Protein RUFY3, Rap2 interacting protein x, Rap2-interacting protein x

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  WB analysis using 83601-2-RR (same clone as 83601-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83601-2-RR (RUFY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83601-2-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83601-2-RR (same clone as 83601-2-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RUFY3 Recombinant antibody (83601-2-RR, Clone:240519E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83601-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00602-2

  Cytometric bead array standard curve of MP00602-2, RUFY3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83601-1-PBS. Detection antibody: 83601-2-PBS. Standard: Ag35558. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 83601-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83601-2-RR against Human RUFY3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83601-2-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA RUFY3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RUFY3 fusion protein Ag35558
• Vollständiger Name: RUN and FYVE domain containing 3
• Berechnetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC051716
• Gene symbol: RUFY3
• Gene ID (NCBI): 22902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.