RUNX1 (N-terminal) Polyklonaler Antikörper

RUNX1 (N-terminal) Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 19555-1-AP

Synonyme

AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1, RUNX1 (N-terminal)

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Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19555-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 19555-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19555-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19555-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19555-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19555-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19555-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19555-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19555-1-AP

  IP Result of anti-RUNX1 (IP:19555-1-AP, 4ug; Detection:19555-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen, HL-60-Zellen, Jurkat-Zellen, Mausmilzgewebe, Maus-Thymusgewebe, MCF-7-Zellen, Raji-Zellen, SH-SY5Y-Zellen, Sp2/0-Zellen
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

19555-1-AP bindet in WB, IP, CoIP, ELISA RUNX1 (N-terminal) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: runt-related transcription factor 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: NM_001754
• Gene symbol: RUNX1
• Gene ID (NCBI): 861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa. This antibody (19555-1-AP) can't recognize 37 kDa isoform 11.

Publikationen

Species	Application	Title
mouse	WB	Protein Expr Purif; Msx1 cooperates with Runx1 for inhibiting myoblast differentiation.; Authors - Guoqiang Zhou
human	WB,CoIP	Mol Cells; RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15; Authors - Hao Ding