RUNX1 (middle) Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RUNX1 (middle) Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (2)

Kat-Nr. : 25315-1-AP

Synonyme

AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1, RUNX1 (middle)

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Galerie der Validierungsdaten

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  WB analysis using 25315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25315-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25315-1-AP (RUNX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25315-1-AP

  IP Result of anti-RUNX1 (IP:25315-1-AP, 4ug; Detection:25315-1-AP 1:300) with Jurkat cells lysate 3440ug.

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  IHC staining of human colon cancer using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25315-1-AP (RUNX1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25315-1-AP (RUNX1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25315-1-AP

  Immunofluorescent analysis of HepG2 cells using 25315-1-AP (RUNX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 25315-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human RUNX1 (middle) (25315-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

25315-1-AP bindet in WB, IP, IHC, IF, FC, ELISA RUNX1 (middle) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RUNX1 (middle) fusion protein Ag17838
• Vollständiger Name: runt-related transcription factor 1
• Berechnetes Molekulargewicht: 480 aa, 52 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC136381
• Gene symbol: RUNX1
• Gene ID (NCBI): 861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa.

Publikationen

Species	Application	Title
mouse	WB	Immunity; The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.; Authors - Dapeng Wang
mouse	IHC, WB	Genes Dev; ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer.; Authors - Rachelle R Olsen
human	WB	J Hematol Oncol; RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.; Authors - Rabindranath Bera
rat	WB	Int Immunopharmacol; miR-9 targeting RUNX1 improves LPS-induced alveolar hypercoagulation and fibrinolysis inhibition through NF-κB inactivation in ARDS; Authors - Chuan Xiao
human	WB	J Cell Physiol; Inhibition of RUNX1 blocks the differentiation of lung fibroblasts to myofibroblasts.; Authors - Shubham Dubey ; KD Validated
human	WB	Eur J Pharmacol; Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells; Authors - Meifang Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SD (Verified Customer) (07-08-2022)

Excellent Antibody!

• Applications: Western Blot
• Cell Tissue Type: H9C2

RUNX1 (middle) Antibody Western Blot validation ( dilution) in H9C2 (Cat no:25315-1-AP)

FH

P (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: fibroblast

RUNX1 (middle) Antibody Western Blot validation ( dilution) in fibroblast (Cat no:25315-1-AP)