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RUNX2 Polyklonaler Antikörper
RUNX2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 20700-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | ROS1728-Zellen, MCF-7-Zellen |
Erfolgreiche IP | ROS1728-Zellen |
Erfolgreiche Detektion in IHC | Rattenhautgewebe, Rattennierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | ROS1728-Zellen |
Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:250-1:1000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 4 publications below |
WB | See 200 publications below |
IHC | See 29 publications below |
IF | See 51 publications below |
IP | See 2 publications below |
ChIP | See 3 publications below |
Produktinformation
20700-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA RUNX2 und zeigt Reaktivität mit human, Ratten
Getestete Reaktivität | human, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | runt-related transcription factor 2 |
Berechnetes Molekulargewicht | 57 kDa |
Beobachtetes Molekulargewicht | 57-60 kDa |
GenBank-Zugangsnummer | NM_004348 |
Gene symbol | RUNX2 |
Gene ID (NCBI) | 860 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for RUNX2 antibody 20700-1-AP | Protokoll herunterladen |
IHC protocol for RUNX2 antibody 20700-1-AP | Protokoll herunterladenl |
IF protocol for RUNX2 antibody 20700-1-AP | Protokoll herunterladen |
IP protocol for RUNX2 antibody 20700-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Bioact Mater 3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration | ||
Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy | ||
Redox Biol Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca2+/ERK pathway-enhanced aberrant osteogenic activity | ||
Mater Today Bio Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration. | ||
J Nanobiotechnology Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway | ||
ACS Appl Mater Interfaces Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH YINGJIAN (Verified Customer) (08-15-2025) | high specificity, strong/robust signal, low background, consistent results, reproducible performance, good lot-to-lot consistency
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