• Featured Product
  • KD/KO Validated

RUNX2 Polyklonaler Antikörper

RUNX2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(235)

Kat-Nr. : 20700-1-AP

Synonyme

AML3, AML-3, CBF-alpha-1, Core-binding factor subunit alpha-1, OSF 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBROS1728-Zellen, MCF-7-Zellen
Erfolgreiche IPROS1728-Zellen
Erfolgreiche Detektion in IHCRattenhautgewebe, Rattennierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCROS1728-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20700-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA RUNX2 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name runt-related transcription factor 2
Berechnetes Molekulargewicht 57 kDa
Beobachtetes Molekulargewicht 57-60 kDa
GenBank-ZugangsnummerNM_004348
Gene symbol RUNX2
Gene ID (NCBI) 860
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for RUNX2 antibody 20700-1-APProtokoll herunterladen
IHC protocol for RUNX2 antibody 20700-1-APProtokoll herunterladenl
IF protocol for RUNX2 antibody 20700-1-APProtokoll herunterladen
IP protocol for RUNX2 antibody 20700-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Bioact Mater

3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration

Authors - Lei Zhang
mouseWB

Small

Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy

Authors - Dan Li
mouseWB

Redox Biol

Endogenous hydrogen sulfide accelerated trauma-induced heterotopic ossification through the Ca2+/ERK pathway-enhanced aberrant osteogenic activity

Authors - Zhengqiang Yuan
humanWB

Mater Today Bio

Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.

Authors - Zhi-Hu Zhao
mouseWB,IF

J Nanobiotechnology

Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway

Authors - Yue Li
WB

ACS Appl Mater Interfaces

Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis

Authors - Congcong Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

YINGJIAN (Verified Customer) (08-15-2025)

high specificity, strong/robust signal, low background, consistent results, reproducible performance, good lot-to-lot consistency

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: heart
RUNX2 Antibody Western Blot validation (1:5000 dilution) in heart (Cat no:20700-1-AP)