RUNX2 Rekombinanter Antikörper

RUNX2 Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

1G5

Rezensionen (2)

Kat-Nr. : 82636-2-RR

Synonyme

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

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• 

  WB analysis using 82636-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82636-2-RR (RUNX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of ROS1728 using 82636-2-RR

  IP result of anti-RUNX2 (IP:82636-2-RR, 4ug; Detection:82636-2-RR 1:500) with ROS1728 cells lysate 2080 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, C2C12-Zellen
• Erfolgreiche IP: ROS1728-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

82636-2-RR bindet in WB, IHC, IF, IP, CoIP, ELISA RUNX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: runt-related transcription factor 2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57-60 kDa
• GenBank-Zugangsnummer: NM_004348
• Gene symbol: RUNX2
• Gene ID (NCBI): 860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.

Publikationen

Species	Application	Title
mouse	WB,IHC	Int Immunopharmacol; Aging and autophagic phenotypic changes in bone marrow mesenchymal stem cells in glucocorticoid-induced osteonecrosis; Authors - Ruomu Cao
mouse	WB	Intractable Rare Dis Res; Role of IFITM2 in osteogenic differentiation of C3H10T1/2 mesenchymal stem cells; Authors - Yongtao Zhang
human	WB	Bone; CTSD upregulation as a key driver of spinal ligament abnormalities in spinal stenosis; Authors - Lei Li
human	WB	Arch Oral Biol; Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis; Authors - Yu Ren
rat	WB	J Orthop Surg Res; Uniaxial static strain enhances osteogenic and angiogenic potential under hypoxic conditions in distraction osteogenesis; Authors - Lifang Zhang
mouse,rat	WB,CoIP	Cell Commun Signal; Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling; Authors - Xue-Xue Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-22-2024)

I used this antibody in WB in cells that differentiate into osteoblast lineage upon stimulation. I had a strong visible band at the expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: C3H10T1/2

RUNX2 Antibody Western Blot validation (1/2000 dilution) in C3H10T1/2 (Cat no:82636-2-RR)

FH

Udesh (Verified Customer) (11-26-2023)

Worked well for ADSCs at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ADSCs