RUVBL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RUVBL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, CHIP, ELISA

Konjugation

Unkonjugiert

Publikationen(49)

Rezensionen (1)

Kat-Nr. : 10210-2-AP

Synonyme

ECP54, ECP 54, EC:3.6.4.12, 49 kDa TBP-interacting protein, 49 kDa TBP interacting protein

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  WB analysis using 10210-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10210-2-AP (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10210-2-AP

  IP result of anti-RUVBL1 (IP:10210-2-AP, 3ug; Detection:10210-2-AP 1:1000) with HeLa cells lysate 800ug.

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  IHC staining of mouse liver using 10210-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10210-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 10210-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 10210-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 10210-2-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RUVBL1 antibody (10210-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 44 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

10210-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, CHIP, ELISA RUVBL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RUVBL1 fusion protein Ag0281
• Vollständiger Name: RuvB-like 1 (E. coli)
• Berechnetes Molekulargewicht: 456 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002993
• Gene symbol: RUVBL1
• Gene ID (NCBI): 8607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.

Publikationen

Species	Application	Title
mouse		Nat Immunol; Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families.; Authors - Yi Zhong
mouse	WB	Mol Cell; Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.; Authors - Sang Gyun Kim
mouse	CHIP	Nat Struct Mol Biol; R loops regulate promoter-proximal chromatin architecture and cellular differentiation.; Authors - Poshen B Chen
human	WB	Sci Adv; Assembly of the asymmetric human γ-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase.; Authors - Fabian Zimmermann ; KD Validated
human	WB	Sci Adv; Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.; Authors - Seung Ho Shin ; KD Validated
human	IP	Genes Dev; SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.; Authors - Ailone Tichon

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

It worked well in H1 human embryonic stem cell.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human embryonic stem cell

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