RXRA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RXRA Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21218-1-PBS

Synonyme

---

• 

  WB analysis of MCF-7 using 21218-1-AP (same clone as 21218-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21218-1-AP (same clone as 21218-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HCT 116 using 21218-1-AP (same clone as 21218-1-PBS)

  HCT 116 lysates prepared and IP of RXRA performed using 1.0 μg of 21218-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 21218-1-AP (same clone as 21218-1-PBS)

  IP result of anti-RXRA (IP:21218-1-AP, 4ug; Detection:21218-1-AP 1:600) with MCF-7 cells lysate 800ug. This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21218-1-AP (same clone as 21218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21218-1-AP (same clone as 21218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 21218-1-AP (same clone as 21218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 21218-1-AP (same clone as 21218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21218-1-AP (same clone as 21218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 21218-1-AP (same clone as 21218-1-PBS)

  Immunofluorescent analysis of Hela cells, using RXRA antibody 21218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21218-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

21218-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA RXRA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RXRA fusion protein Ag15651
• Vollständiger Name: retinoid X receptor, alpha
• Berechnetes Molekulargewicht: 462 aa, 51 kDa
• Beobachtetes Molekulargewicht: 50-54 kDa
• GenBank-Zugangsnummer: BC110998
• Gene symbol: RXRA
• Gene ID (NCBI): 6256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver and also expressed in the lungs, kidneys, and heart. It can recognize the mature 54 kDa RXRA and the truncated 44 kDa RXRA (PMID: 20541701).