RXRA Monoklonaler Antikörper

RXRA Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

4H6C4

Kat-Nr. : 60198-1-Ig

Synonyme

NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA, RXRα

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  WB analysis of HeLa using 60198-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60198-1-Ig (RXRA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, Maus-Cerebellum-Gewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

60198-1-Ig bindet in WB, IHC, ELISA RXRA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RXRA fusion protein Ag0987
• Vollständiger Name: retinoid X receptor, alpha
• Berechnetes Molekulargewicht: 462 aa, 51 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC007925
• Gene symbol: RXRA
• Gene ID (NCBI): 6256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver, and also expressed in the lungs, kidneys, and heart. It can recognize the the mature 54 kDa RXRA and the truncated 44 kD RXRA (PMID: 20541701).

Publikationen

Species	Application	Title
human	WB	Int J Endocrinol; Constitutive Androstane Receptor-Mediated Inhibition of Metformin on Phase II Metabolic Enzyme SULT2A1; Authors - Xiaowen Hu
mouse	WB	Exp Mol Med; LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver; Authors - Ming Jin