RXRA Rekombinanter Antikörper

RXRA Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

240878A9

Kat-Nr. : 83796-5-PBS

Synonyme

ORF-3, 240878A9, NR2B1, ORF3, Retinoic acid receptor RXR-alpha

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  WB analysis using 83796-5-RR (same clone as 83796-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83796-5-RR (RXRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83796-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83796-5-RR (same clone as 83796-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RXRA antibody (83796-5-RR, Clone: 240878A9 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83796-5-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83796-5-RR (same clone as 83796-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RXRA Recombinant antibody (83796-5-RR, Clone:240878A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83796-5-PBS in a different storage buffer formulation.

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  ChIP experiment of HepG2 using 83796-5-RR (same clone as 83796-5-PBS)

  Chromatin was prepared from HepG2 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of RXRA (83796-5-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83796-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83796-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83796-5-RR against Human RXRA were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83796-5-PBS bindet in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR RXRA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RXRA fusion protein Ag15651
• Vollständiger Name: retinoid X receptor, alpha
• Berechnetes Molekulargewicht: 462 aa, 51 kDa
• Beobachtetes Molekulargewicht: 54 kDa, 44 kDa
• GenBank-Zugangsnummer: BC110998
• Gene symbol: RXRA
• Gene ID (NCBI): 6256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver and also expressed in the lungs, kidneys, and heart. It can recognize the mature 54 kDa RXRA and the truncated 44 kDa RXRA (PMID: 20541701).