RXRB Polyklonaler Antikörper

RXRB Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 14684-1-AP

Synonyme

DAUDI6, H 2RIIBP, NR2B2, RCoR 1, Retinoid X receptor beta, retinoid X receptor, beta, RXRB

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  WB analysis of NIH/3T3 using 14684-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14684-1-AP (RXRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14684-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14684-1-AP (RXRB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14684-1-AP

  IP result of anti-RXRB (IP:14684-1-AP, 4ug; Detection:14684-1-AP 1:500) with MCF-7 cells lysate 1200ug.

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  IHC staining of human colon cancer using 14684-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14684-1-AP (RXRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14684-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14684-1-AP (RXRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

14684-1-AP bindet in WB, IHC, IF, IP, ELISA RXRB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RXRB fusion protein Ag0263
• Vollständiger Name: retinoid X receptor, beta
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa, 70 kDa
• GenBank-Zugangsnummer: BC001167
• Gene symbol: RXRB
• Gene ID (NCBI): 6257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)

Publikationen

Species	Application	Title
human	WB	Autophagy; Neutrophil-derived serine proteases induce FOXA2-mediated autophagy dysfunction and exacerbate colitis-associated carcinogenesis via F2RL1/protease-activated receptor 2; Authors - Junhu Yuan
rat	IF	Am J Physiol Lung Cell Mol Physiol; Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.; Authors - Craig A Gelfand
mouse	WB	Exp Mol Med; LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver; Authors - Ming Jin
rat	WB	Mol Neurobiol; Verification of Pain-Related Neuromodulation Mechanisms of Calcitonin in Knee Osteoarthritis; Authors - Qing Lin