RXRB Rekombinanter Antikörper

RXRB Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242178D4

Kat-Nr. : 84656-4-PBS

Synonyme

242178D4, DAUDI6, H 2RIIBP, NR2B2, RCoR 1

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  WB analysis using 84656-4-RR (same clone as 84656-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84656-4-RR (RXRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84656-4-RR (same clone as 84656-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84656-4-RR (RXRB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84656-4-RR (same clone as 84656-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84656-4-RR (RXRB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84656-4-RR (same clone as 84656-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RXRB antibody (84656-4-RR, Clone: 242178D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84656-4-RR (same clone as 84656-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RXRB antibody (84656-4-RR, Clone: 242178D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84656-4-RR (same clone as 84656-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RXRB Recombinant antibody (84656-4-RR, Clone:242178D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84656-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84656-4-RR against Human RXRB were performed. The affinity constant is 1.24 nM.

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Geprüfte Anwendungen

Produktinformation

84656-4-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA RXRB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RXRB fusion protein Ag0263
• Vollständiger Name: retinoid X receptor, beta
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC001167
• Gene symbol: RXRB
• Gene ID (NCBI): 6257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor, and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)