RXRG Polyklonaler Antikörper

RXRG Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, PLA, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11129-1-AP

Synonyme

NR2B3, Retinoid X receptor gamma, retinoid X receptor, gamma, RXRC, RXRG

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 11129-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse ovary using 11129-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11129-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat eye using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat eye using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Jurkat-Zellen, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: Ratten-Augengewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11129-1-AP bindet in WB, PLA, IHC, IF, ELISA RXRG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RXRG fusion protein Ag1611
• Vollständiger Name: retinoid X receptor, gamma
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51-55 kDa
• GenBank-Zugangsnummer: BC012063
• Gene symbol: RXRG
• Gene ID (NCBI): 6258
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RXRG, also named as, Retinoic acid receptor RXR-gamma, is a 463 amino acid protein, which localize in the nucleus and may form homodimer. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5.

Publikationen

Species	Application	Title
mouse	PLA	Aging (Albany NY); Cholecalciferol (vitamin D3) has a direct protective activity against interleukin 6-induced atrophy in C2C12 myotubes.; Authors - Maraiza Alves Teixeira
rat	IF	Am J Physiol Lung Cell Mol Physiol; Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.; Authors - Craig A Gelfand
human	WB	Cells; EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP.; Authors - Emanuela Nocita