RYBP Monoklonaler Antikörper

RYBP Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C6G7

Kat-Nr. : 68130-1-PBS

Synonyme

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

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  WB analysis using 68130-1-Ig (same clone as 68130-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68130-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 68130-1-Ig (same clone as 68130-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68130-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68130-1-PBS bindet in WB, Indirect ELISA RYBP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RYBP fusion protein Ag31715
• Vollständiger Name: RING1 and YY1 binding protein
• Berechnetes Molekulargewicht: 228 aa, 25 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC014959
• Gene symbol: RYBP
• Gene ID (NCBI): 23429
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]