RYK Polyklonaler Antikörper

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• KD/KO Validated

RYK Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 22138-1-AP

Synonyme

D3S3195, EC:2.7.10.1, JTK5, JTK5A, RYK1

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  WB analysis of A549 using 22138-1-AP

  WB result of RYK antibody (22138-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RYK transfected A549 cells.

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  WB analysis using 22138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A549-Zellen, Caco-2-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

22138-1-AP bindet in WB, ELISA RYK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RYK fusion protein Ag17540
• Vollständiger Name: RYK receptor-like tyrosine kinase
• Berechnetes Molekulargewicht: 610 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC153090
• Gene symbol: RYK
• Gene ID (NCBI): 6259
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tyrosine-protein kinase RYK may be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A, and WNT5A. It is involved in neuron differentiation, axon guidance, corpus callosum establishment, and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region, allowing the C-terminal intracellular product to translocate from the cytoplasm to the nucleus, which plays a crucial role in neuronal development.

Publikationen

Species	Application	Title
mouse	WB	J Neuroinflammation; Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model; Authors - Yitian Lu
human	WB	Cell Mol Biol Lett; Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway; Authors - Zhaoyi Liang
human	WB	Pharmaceuticals (Basel); RYK Gene Expression Associated with Drug Response Variation of Temozolomide and Clinical Outcomes in Glioma Patients; Authors - Ricardo D Gonzalez