RYR1 Polyklonaler Antikörper

RYR1 Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26968-1-AP

Synonyme

CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, RYR1/2/3, SKRR

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  IHC staining of mouse brain using 26968-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 26968-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 26968-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 26968-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cerebellum using 26968-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 26968-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 26968-1-AP (RYR1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse cerebellum using 26968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RYR1 antibody (26968-1-AP) at dilution of 1:200 and CoraLite®647-conjugated F(ab.

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  IF Staining of mouse brain using 26968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RYR1 antibody (26968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Cerebellum-Gewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Cerebellum-Gewebe, Maushirngewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

26968-1-AP bindet in WB, IHC, IF-P, ELISA RYR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RYR1 fusion protein Ag25120
• Vollständiger Name: ryanodine receptor 1 (skeletal)
• Berechnetes Molekulargewicht: 565 kDa
• GenBank-Zugangsnummer: NM_000540
• Gene symbol: RYR1
• Gene ID (NCBI): 6261
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.

Publikationen

Species	Application	Title
mouse	IF	JCI Insight; Muscle specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism; Authors - Benedict Abdon
chicken	WB	Poult Sci; Effects of chronic heat stress on Ca2+ homeostasis, apoptosis, and protein carbonylation profiles in the breast muscle of broilers; Authors - Yingsen Liu