• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RYR1 Monoklonaler Antikörper

RYR1 Monoklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B4E12

Kat-Nr. : 66539-1-PBS

Synonyme

1B4E12, CCO, Ryanodine receptor 1, RYDR, RYR1/2/3

Filter:
  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using RYR1 Monoclonal antibody (66539-1-Ig)

    IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using RYR1 Monoclonal antibody (66539-1-Ig)

    IHC staining of mouse skeletal muscle using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RYR1 Monoclonal antibody (66539-1-Ig)

    IHC staining of mouse cerebellum using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66539-1-Ig (RYR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A-673 cells using RYR1 Monoclonal antibody (66539-1-Ig)

    IF Staining of A-673 using 66539-1-Ig (same clone as 66539-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A-673 cells using RYR1 antibody (66539-1-Ig, Clone: 1B4E12 ) at dilution of 1:300 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66539-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66539-1-PBS bindet in IHC, IF/ICC, Indirect ELISA RYR1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen RYR1 fusion protein Ag25120
Vollständiger Name ryanodine receptor 1 (skeletal)
Berechnetes Molekulargewicht 565 kDa
GenBank-ZugangsnummerNM_000540
Gene symbol RYR1
Gene ID (NCBI) 6261
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.