RYR2 Polyklonaler Antikörper

RYR2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 19765-1-AP

Synonyme

ryanodine receptor 2, cardiac, ryanodine receptor 2 (cardiac), hRYR-2, Cardiac muscle ryanodine receptor-calcium release channel, ARVD2

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  WB analysis using 19765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19765-1-AP (RYR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human skeletal muscle using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 19765-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19765-1-AP (Ryanodine Receptor 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse heart using 19765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (19765-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 19765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (19765-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 19765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (19765-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 19765-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 19765-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 19765-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 19765-1-AP (Ryanodine Receptor 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hirngewebe, humanes Plazenta-Gewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

19765-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA RYR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ryanodine receptor 2 (cardiac)
• Berechnetes Molekulargewicht: 565 kDa
• GenBank-Zugangsnummer: NM_001035
• Gene symbol: RYR2
• Gene ID (NCBI): 6262
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP). This antibody detects a band at ~250 kDa which has been reported (PMID: 22962011).

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Subcellular activation of β-adrenergic receptors using a spatially restricted antagonist; Authors - Federica Liccardo
mouse	WB	Sci China Life Sci; LncRNA CCRR maintains Ca2+ homeostasis against myocardial infarction through the FTO-SERCA2a pathway; Authors - Hua Yang
mouse	WB	Mol Metab; Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor; Authors - Kelsey M Pinckard
mouse	WB	Cell Mol Life Sci; Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip-Trx2 signaling; Authors - Zhi-Xing Wei
human,mouse	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen ; KD Validated
rat	WB	Part Fibre Toxicol; Oxygenated polycyclic aromatic hydrocarbons from ambient particulate matter induce electrophysiological instability in cardiomyocytes.; Authors - Sujin Ju