RYR2 Polyklonaler Antikörper

RYR2 Polyklonal Antikörper für IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27587-1-PBS

Synonyme

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  IHC staining of human heart using 27587-1-AP (same clone as 27587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27587-1-AP (RYR2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 27587-1-AP (same clone as 27587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27587-1-AP (RYR2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 27587-1-AP (same clone as 27587-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (27587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 27587-1-AP (same clone as 27587-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (27587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 27587-1-AP (same clone as 27587-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human RYR2 (27587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27587-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27587-1-PBS bindet in IHC, IF-P, FC (Intra), Indirect ELISA RYR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RYR2 fusion protein Ag26296
• Vollständiger Name: ryanodine receptor 2 (cardiac)
• Berechnetes Molekulargewicht: 565 kDa
• GenBank-Zugangsnummer: NM_001035
• Gene symbol: RYR2
• Gene ID (NCBI): 6262
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP).