RanBP1 Polyklonaler Antikörper

RanBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27804-1-PBS

Synonyme

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  WB analysis using 27804-1-AP (same clone as 27804-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27804-1-AP (same clone as 27804-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 27804-1-AP (same clone as 27804-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 27804-1-AP (same clone as 27804-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 27804-1-AP (same clone as 27804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27804-1-AP (RanBP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 27804-1-AP (same clone as 27804-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 27804-1-AP (same clone as 27804-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27804-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27804-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RanBP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RanBP1 fusion protein Ag25714
• Vollständiger Name: RAN binding protein 1
• Beobachtetes Molekulargewicht: 28 kDa
• Gene symbol: RANBP1
• Gene ID (NCBI): 5902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RanBP1 (Ran-binding protein 1) belongs to the RANBP1 family. RanBP1 plays a role in RAN-dependent nucleocytoplasmic transport. It alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. And RanBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RanBP1 promotes dissociation of RAN from a complex with KPNA2 and CSE1L. It required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PMID:17671426). Also, RanBP1 inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PMID:7882974, PMID:7616957).