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RanBP2 Polyklonaler Antikörper

RanBP2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27606-1-PBS

Synonyme

358 kDa nucleoporin, ANE1, E3 SUMO protein ligase RanBP2, Nucleoporin Nup358, NUP358

Filter:
  • Western Blot (WB) analysis of various lysates using RanBP2 Polyclonal antibody (27606-1-AP)

    WB analysis using 27606-1-AP (same clone as 27606-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using RanBP2 Polyclonal antibody (27606-1-AP)

    IHC staining of rat liver using 27606-1-AP (same clone as 27606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27606-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RanBP2 Polyclonal antibody (27606-1-AP)

    IHC staining of mouse kidney using 27606-1-AP (same clone as 27606-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27606-1-AP (RanBP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27606-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27606-1-PBS bindet in WB, IHC, Indirect ELISA RanBP2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RanBP2 fusion protein Ag26370
Vollständiger Name RAN binding protein 2
Berechnetes Molekulargewicht 358 kDa
Beobachtetes Molekulargewicht 358 kDa
GenBank-ZugangsnummerNM_006267
Gene symbol RANBP2
Gene ID (NCBI) 5903
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RanBP2 (Ran-binding protein 2) is also named as p270 and NUP358, and belongs to the RanBP2 E3 ligase family. RanBP2 is E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PMID:11792325, PMID:22194619). RanBP2 involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PMID:7775481). It is component of the nuclear export pathway (PMID:10078529). RanBP2 recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PMID:20386726).