RanGAP1 Polyklonaler Antikörper

RanGAP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27405-1-AP

Synonyme

SD, Ran GTPase-activating protein 1, KIAA1835

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  WB analysis of SKOV-3 using 27405-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27405-1-AP (RanGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 27405-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 27405-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27405-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27405-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 27405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27405-1-AP (RanGAP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, MCF-7-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

27405-1-AP bindet in WB, IHC, IF, ELISA RanGAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RanGAP1 fusion protein Ag26128
• Vollständiger Name: Ran GTPase activating protein 1
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa, 82 kDa
• GenBank-Zugangsnummer: BC014044
• Gene symbol: RANGAP1
• Gene ID (NCBI): 5905
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
mouse	WB,IF	iScience; Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain; Authors - Terry R Suk