Raptor Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Raptor Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20984-1-PBS

Synonyme

KIAA1303, TOR, RPTOR, Regulatory-associated protein of mTOR, p150 target of rapamycin

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  WB analysis of HepG2 cells using 20984-1-AP (same clone as 20984-1-PBS)

  WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  WB analysis using 20984-1-AP (same clone as 20984-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20984-1-AP (Raptor antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 20984-1-AP (same clone as 20984-1-PBS)

  IP result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:4000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 20984-1-AP (same clone as 20984-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Raptor antibody (20984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20984-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20984-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA Raptor und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: raptor
• Berechnetes Molekulargewicht: 149 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: NM_020761
• Gene symbol: Raptor
• Gene ID (NCBI): 57521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.