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Raptor Monoklonaler Antikörper

Raptor Monoklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B8A12

Kat-Nr. : 68286-1-PBS

Synonyme

KIAA1303, raptor, RPTOR

Filter:
  • Western Blot (WB) analysis of various lysates using Raptor Monoclonal antibody (68286-1-Ig)

    WB analysis using 68286-1-Ig (same clone as 68286-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68286-1-Ig (Raptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68286-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Raptor Monoclonal antibody (68286-1-Ig)

    IP experiment of HeLa using 68286-1-Ig (same clone as 68286-1-PBS)

    IP result of anti-Raptor (IP:68286-1-Ig, 4ug; Detection:68286-1-Ig 1:1000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 68286-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68286-1-PBS bindet in WB, IP, Indirect ELISA Raptor und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Raptor fusion protein Ag30131
Vollständiger Name raptor
Berechnetes Molekulargewicht 1335 aa, 149 kDa
Beobachtetes Molekulargewicht 130-150 kDa
GenBank-ZugangsnummerBC136654
Gene symbol Raptor
Gene ID (NCBI) 57521
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.