S100A10 Monoklonaler Antikörper

S100A10 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3F9

Kat-Nr. : 66227-1-PBS

Synonyme

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

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  WB analysis of human lung using 66227-1-Ig (same clone as 66227-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 66227-1-Ig (S100A10 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66227-1-Ig (S100A10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66227-1-Ig (S100A10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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  IF Staining of Saos-2 using 66227-1-Ig (same clone as 66227-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using S100A10 antibody (66227-1-Ig, Clone: 1B3F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66227-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66227-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA S100A10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S100A10 fusion protein Ag23596
• Vollständiger Name: S100 calcium binding protein A10
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC015973
• Gene symbol: S100A10
• Gene ID (NCBI): 6281
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.