S100A11 Monoklonaler Antikörper

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• KD/KO Validated

S100A11 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A3F1

Kat-Nr. : 60024-1-PBS

Synonyme

Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C, S100A11, S100C

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  WB analysis of T-47D using 60024-1-Ig (same clone as 60024-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60024-1-Ig (same clone as 60024-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 60024-1-Ig (same clone as 60024-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 60024-1-Ig (same clone as 60024-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using S100A11 antibody (60024-1-Ig, Clone: 1A3F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60024-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA S100A11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S100A11 fusion protein Ag0343
• Vollständiger Name: S100 calcium binding protein A11
• Berechnetes Molekulargewicht: 105 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC001410
• Gene symbol: S100A11
• Gene ID (NCBI): 6282
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A11, also named as MLN 70, Calgizzarin and S100C, belongs to the S-100 family. It facilitates the differentiation and the cornification of keratinocytes. The naming of S100A11 systematically arises from its membership of the S100 protein family, named after their solubility in 100% saturated ammonium sulfate solution. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation.