S100A12 Polyklonaler Antikörper

S100A12 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 16630-1-AP

Synonyme

CAAF1, CAGC, Calgranulin C, CGRP, EN RAGE, ENRAGE, MRP6, Neutrophil S100 protein, p6, Protein S100 A12, S100A12

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  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

16630-1-AP bindet in WB, IHC, ELISA S100A12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A12 fusion protein Ag9961
• Vollständiger Name: S100 calcium binding protein A12
• Berechnetes Molekulargewicht: 92 aa, 11 kDa
• GenBank-Zugangsnummer: BC070294
• Gene symbol: S100A12
• Gene ID (NCBI): 6283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A12 is expressed abundantly in granulocytes, and has been implicated to play an important role on inflammatory reactions in various disease states.

Publikationen

Species	Application	Title
	IHC	Biol Reprod; Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling.; Authors - Claire Stenhouse
human	WB,IHC	Connect Tissue Res; S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway; Authors - Yi Zhang
human	IHC	Redox Biol; Lipoxin A4 ameliorates knee osteoarthritis progression in rats by antagonizing ferroptosis through activation of the ESR2/LPAR3/Nrf2 axis in synovial fibroblast-like synoviocytes; Authors - Zhehan Hu
	WB	Animals (Basel); Comparative Study on the Sperm Proteomes of Horses and Donkeys; Authors - Hong Ren
human	WB	Front Immunol; Identification and validation of neutrophil-related biomarkers in acute-on-chronic liver failure; Authors - Wei Lin