S100A16 Polyklonaler Antikörper

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• KD/KO Validated

S100A16 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11456-1-PBS

Synonyme

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• 

  WB analysis using 11456-1-AP (same clone as 11456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 11456-1-AP (same clone as 11456-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 11456-1-AP (same clone as 11456-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11456-1-AP (S100A16 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11456-1-AP (same clone as 11456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 11456-1-AP (same clone as 11456-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using S100A16 antibody (11456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11456-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11456-1-PBS bindet in WB, IHC, IF/ICC, ELISA S100A16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A16 fusion protein Ag2023
• Vollständiger Name: S100 calcium binding protein A16
• Berechnetes Molekulargewicht: 103 aa, 12 kDa
• Beobachtetes Molekulargewicht: 9-12 kDa
• GenBank-Zugangsnummer: BC019099
• Gene symbol: S100A16
• Gene ID (NCBI): 140576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).