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S100A4 Monoklonaler Antikörper
S100A4 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
2G11B4
Kat-Nr. : 66489-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | NK-92 cells, A375-Zellen, A549-Zellen, HeLa-Zellen, NIH/3T3-Zellen, PC-3-Zellen, THP-1-Zellen |
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Tonsillitisgewebe, K-562-Zellen, Mauslungengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 6 publications below |
| IF | See 16 publications below |
| ELISA | See 1 publications below |
| FC | See 1 publications below |
Produktinformation
66489-1-Ig bindet in WB, IHC, IF/ICC, ELISA S100A4 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | S100A4 fusion protein Ag9019 |
| Vollständiger Name | S100 calcium binding protein A4 |
| Berechnetes Molekulargewicht | 101 aa, 12 kDa |
| Beobachtetes Molekulargewicht | 12 kDa |
| GenBank-Zugangsnummer | BC016300 |
| Gene symbol | S100A4 |
| Gene ID (NCBI) | 6275 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for S100A4 antibody 66489-1-Ig | Protokoll herunterladen |
| IHC protocol for S100A4 antibody 66489-1-Ig | Protokoll herunterladenl |
| IF protocol for S100A4 antibody 66489-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse | ||
Adv Sci (Weinh) Meis1 Targets Protein Tyrosine Phosphatase Receptor J in Fibroblast to Retard Chronic Kidney Disease Progression | ||
Cell Death Differ Upregulation of Wilms' Tumor 1 in epicardial cells increases cardiac fibrosis in dystrophic mice. | ||
Acta Pharmacol Sin PU.1/Spi1 exacerbates ischemia-reperfusion induced acute kidney injury via upregulating Gata2 and promoting fibroblast activation | ||
PLoS Pathog S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation. | ||
Cancers (Basel) Usp22 Overexpression Leads to Aberrant Signal Transduction of Cancer-Related Pathways but Is Not Sufficient to Drive Tumor Formation in Mice |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH CAITLIN (Verified Customer) (03-04-2025) | Worked great, highly specific even when used at higher dilution than recommended
|
FH Caitlin (Verified Customer) (10-15-2024) | Works well, very convenient that the antibody can be used at such high dilutions and still achieve band specificity!
|
FH Shinford (Verified Customer) (10-26-2023) | I have some reservations about the antibody because of its relatively low molecular weight. We attempted to create separate gels with 10% and 12% concentrations and made an effort to run the gels for a limited time to ensure that the entire protein remained within the original gels and membranes. While we were able to detect several bands, their positions lower in the gel led to less than ideal band shapes.
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