S100A4 Rekombinanter Antikörper

S100A4 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241448A12

Kat-Nr. : 84221-1-PBS

Synonyme

241448A12, Calvasculin, CAPL, Fibroblast Specific Protein, Fibroblast-specific protein 1

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  WB analysis using 84221-1-RR (same clone as 84221-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84221-1-RR (S100A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84221-1-RR (same clone as 84221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84221-1-RR (S100A4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84221-1-RR (same clone as 84221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84221-1-RR (S100A4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84221-1-RR (same clone as 84221-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84221-1-RR (S100A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84221-1-RR (same clone as 84221-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100A4 antibody (84221-1-RR, Clone: 241448A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84221-1-RR (same clone as 84221-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A4 Recombinant antibody (84221-1-RR, Clone:241448A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 84221-1-RR (same clone as 84221-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A4 Recombinant antibody (84221-1-RR, Clone:241448A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84221-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01155-2

  Cytometric bead array standard curve of MP01155-2, S100A4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84221-2-PBS. Detection antibody: 84221-1-PBS. Standard: Ag9019. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84221-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84221-1-RR against Human S100A4 were performed. The affinity constant is 2.75 nM.

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Geprüfte Anwendungen

Produktinformation

84221-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA S100A4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: S100A4 fusion protein Ag9019
• Vollständiger Name: S100 calcium binding protein A4
• Berechnetes Molekulargewicht: 101 aa, 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa
• GenBank-Zugangsnummer: BC016300
• Gene symbol: S100A4
• Gene ID (NCBI): 6275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.