S100A5 Polyklonaler Antikörper

S100A5 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17924-1-AP

Synonyme

Protein S 100D, Protein S100 A5, S100A5, S100D

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 17924-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 17924-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17924-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 17924-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human brain using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17924-1-AP (S100A5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17924-1-AP (S100A5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17924-1-AP

  Immunofluorescent analysis of HepG2 cells using 17924-1-AP (S100A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe, humanes Nierengewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Meningiom-Gewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

17924-1-AP bindet in WB, IHC, IF, ELISA S100A5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A5 fusion protein Ag12342
• Vollständiger Name: S100 calcium binding protein A5
• Berechnetes Molekulargewicht: 110 aa, 13 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC093955
• Gene symbol: S100A5
• Gene ID (NCBI): 6276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC,IF	Adv Sci (Weinh); S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma; Authors - Huihuang Li
mouse	IHC	Mol Cell Proteomics; The Membrane Proteome of Sensory Cilia to the Depth of Olfactory Receptors.; Authors - Katja Kuhlmann