S100A7/Psoriasin Polyklonaler Antikörper

S100A7/Psoriasin Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26656-1-AP

Synonyme

Protein S100 A7, PSOR1, Psoriasin, S100A7, S100A7/Psoriasin, S100A7c

---

Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 26656-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-468 using 26656-1-AP

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 26656-1-AP (S100A7/Psoriasin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 26656-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 26656-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26656-1-AP (S100A7/Psoriasin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, MDA-MB-468-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26656-1-AP bindet in WB, IHC, ELISA S100A7/Psoriasin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A7/Psoriasin fusion protein Ag24717
• Vollständiger Name: S100 calcium binding protein A7
• Berechnetes Molekulargewicht: 101 aa, 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC034687
• Gene symbol: S100A7
• Gene ID (NCBI): 6278
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A7 (psoriasin), a member of the S100 protein family, is a well-known antimicrobial peptide and a signaling molecule which regulates cellular function and is expressed in many squamous cell carcinomas (SCCs), such as SCC of the skin. It is an EF-hand type calcium binding protein localized in epithelial cells, regulates cell proliferation and differentiation. The protein is overexpressed in hyperproliferative skin diseases, exhibits antimicrobial activities against bacteria and induces immunomodulatory activities.