S100A8 Polyklonaler Antikörper

S100A8 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32681-1-AP

Synonyme

Caga, Calgranulin-A, Chemotactic cytokine CP-10, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8

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  WB analysis using 32681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32681-1-AP (S100a8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 32681-1-AP

  IP result of anti-S100A8 (IP:32681-1-AP, 4ug; Detection:32681-1-AP 1:3000) with mouse spleen tissue lysate 2480 ug.

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  IF Staining of NIH/3T3 using 32681-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using S100A8 antibody (32681-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenmilzgewebe, Mausmilzgewebe
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32681-1-AP bindet in WB, IF/ICC, IP, ELISA S100A8 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A8 fusion protein Eg2959
• Vollständiger Name: S100 calcium binding protein A8 (calgranulin A)
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_013650.2
• Gene symbol: S100a8
• Gene ID (NCBI): 20201
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).