S100A9 Monoklonaler Antikörper

S100A9 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E12C1

Kat-Nr. : 68900-1-Ig

Synonyme

1E12C1, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit

---

• 

  WB analysis of human saliva using 68900-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-468 using 68900-1-Ig

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1376 using 68900-1-Ig

  HT-1376 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SK-BR-3 using 68900-1-Ig

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 68900-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using S100A9 antibody (68900-1-Ig, Clone: 1E12C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human saliva, MDA-MB-468-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68900-1-Ig bindet in WB, IF/ICC, ELISA S100A9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S100A9 fusion protein Ag27025
• Vollständiger Name: S100 calcium binding protein A9
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC047681
• Gene symbol: S100A9
• Gene ID (NCBI): 6280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.