S100A9 Rekombinanter Antikörper

S100A9 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240539G9

Kat-Nr. : 83578-2-PBS

Synonyme

240539G9, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit

---

• 

  WB analysis of MCF-7 using 83578-2-RR (same clone as 83578-2-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 83578-2-PBS

  Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-PBS (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83578-2-RR (same clone as 83578-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83578-2-RR (S100A9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 83578-2-RR (same clone as 83578-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83578-2-RR (same clone as 83578-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A9 antibody (83578-2-RR, Clone: 240539G9 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83578-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83578-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83578-2-PBS against Human S100A9 was performed. The affinity constant is 0.389 nM.

• 

  Affinity and Kinetic Characterization of 83578-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83578-2-RR against Human S100A9 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83578-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA S100A9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: S100A9 fusion protein Ag25764
• Vollständiger Name: S100 calcium binding protein A9
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC047681
• Gene symbol: S100A9
• Gene ID (NCBI): 6280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.