S100A9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S100A9 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-26992

Synonyme

CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit, CFAG

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  IF Staining of HeLa using CL594-26992

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 S100A9 antibody (CL594-26992) at dilution of 1:200.

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  IF Staining of A549 using CL594-26992

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 S100A9 antibody (CL594-26992) at dilution of 1:200.

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  IF Staining of U2OS using CL594-26992

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite®594 S100A9 antibody (CL594-26992) at dilution of 1:200.

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  FC experiment of HeLa using CL594-26992

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite®594 S100a9 Polyclonal Antibody (CL594-26992)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A549-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-26992 bindet in IF/ICC, FC (Intra) S100A9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100A9 fusion protein Ag25764
• Vollständiger Name: S100 calcium binding protein A9
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC047681
• Gene symbol: S100A9
• Gene ID (NCBI): 6280
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer with an S100A8 partner (S100A8/A9), or as a heterotetramer with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.