S1PR2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

S1PR2 Polyklonal Antikörper für WB, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(57)

Rezensionen (1)

Kat-Nr. : 21180-1-AP

Synonyme

AGR16, EDG 5, EDG5, Endothelial differentiation G-protein coupled receptor 5, Gpcr13

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  WB analysis using 21180-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21180-1-AP (S1PR2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 21180-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using S1PR2 antibody (21180-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 21180-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed frozen OCT-embedded mouse brain tissue using 21180-1-AP (S1PR2 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HuH-7-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 48 publications below
• IHC: See 10 publications below
• IF: See 13 publications below
• IP: See 2 publications below

Produktinformation

21180-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA S1PR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S1PR2 fusion protein Ag15470
• Vollständiger Name: sphingosine-1-phosphate receptor 2
• Berechnetes Molekulargewicht: 353 aa, 39 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC069598
• Gene symbol: S1PR2
• Gene ID (NCBI): 9294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S1PR2 (sphingosine-1-phosphate receptor 2), also known as S1P2 or EDG5, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P), a biologically active metabolic product of sphingolipid (PMID: 18787560). S1P and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR2 plays a role in acute vascular inflammation (PMID: 23723450). S1PR2 signaling is implicated in STAT3 activation (PMID: 22606352).

Publikationen

Species	Application	Title
mouse	WB	Gastroenterology; Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells; Authors - Decai Wang
mouse	WB,IP,IHC	Brain; Gamma subunit of complement component 8 is a neuroinflammation inhibitor.; Authors - Jong-Heon Kim
mouse,human	IHC,IF	Nat Commun; Critical role of sphingosine-1-phosphate receptor-2 in the disruption of cerebrovascular integrity in experimental stroke.; Authors - Gab Seok Kim ; KO Validated
human	WB	EMBO Mol Med; Therapeutic development of group B Streptococcus meningitis by targeting a host cell signaling network involving EGFR.; Authors - Ningyu Zhu ; KO Validated
mouse	WB	Theranostics; Endothelial S1pr2 regulates post-ischemic angiogenesis via AKT/eNOS signaling pathway.; Authors - Caixia Zhou ; KO Validated
human	IHC	Front Immunol; Altered sphingolipid pathway in SARS-CoV-2 infected human lung tissue; Authors - Rabisa J Khan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Edma (Verified Customer) (03-03-2025)

The antibody quite nice in western blot, but I have to test it in other cell types

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells