S1PR5/EDG8 Polyklonaler Antikörper

S1PR5/EDG8 Polyklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13874-1-AP

Synonyme

Edg 8, EDG8, S1P receptor 5, S1P receptor Edg 8, S1P5, S1PR5, SPPR 1, SPPR 2

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 13874-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13874-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13874-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13874-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13874-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  FC experiment of SH-SY5Y using 13874-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug S1PR5/EDG8 antibody (13874-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13874-1-AP bindet in WB, FC, ELISA S1PR5/EDG8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S1PR5/EDG8 fusion protein Ag4928
• Vollständiger Name: sphingosine-1-phosphate receptor 5
• Berechnetes Molekulargewicht: 398 aa, 42 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC034703
• Gene symbol: S1PR5
• Gene ID (NCBI): 53637
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that elicits diverse physiological effect on most types of cells and tissues via five G protein-coupled receptors, S1PR1 to S1PR5. S1PRs differ in their tissue expression and cell lineage: S1PR1-3 are expressed throughout the immune, central nervous system (CNS), and cardiovascular system; S1PR4 is primarily expressed in lymphoid tissue; and S1PR5 is a predominant receptor in CNS white matter, spleen and natural killer cells (PMID: 21707490). S1PR5 (also known as EDG8) has been involved in the trafficking of NK cells and monocytes (PMID: 21911833; 23519784).

Publikationen

Species	Application	Title
human	WB	PLoS One; Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2.; Authors - Rie Matsushima-Nishiwaki
mouse	WB	Drug Des Devel Ther; Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.; Authors - Dianna Liu

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