S6 Ribosomal protein Monoklonaler Antikörper

S6 Ribosomal protein Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1C3E10

Kat-Nr. : 66886-1-PBS

Synonyme

RPS6, S6, 1C3E10, 40S ribosomal protein S6, OK/SW-cl.2

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  WB analysis using 66886-1-Ig (same clone as 66886-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66886-1-Ig (same clone as 66886-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66886-1-Ig (same clone as 66886-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 66886-1-Ig (same clone as 66886-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66886-1-Ig (same clone as 66886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66886-1-Ig (same clone as 66886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 66886-1-Ig (same clone as 66886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 66886-1-Ig (same clone as 66886-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66886-1-Ig (same clone as 66886-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S6 Ribosomal protein antibody (66886-1-Ig, Clone: 1C3E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66886-1-Ig (same clone as 66886-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human S6 Ribosomal protein (66886-1-Ig, Clone:1C3E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66886-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA S6 Ribosomal protein und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: S6 Ribosomal protein fusion protein Ag6599
• Vollständiger Name: ribosomal protein S6
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29-32 kDa
• GenBank-Zugangsnummer: BC000524
• Gene symbol: RPS6
• Gene ID (NCBI): 6194
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publikationen

Species	Application	Title
human	WB	Transl Oncol; Next-generation sequencing in early-stage multiple primary lung cancer: The prognostic significance of genomic accumulation status and BCL2L11del; Authors - Mu-Ting Wang