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S6 Ribosomal protein Rekombinanter Antikörper

S6 Ribosomal protein Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6L9

Kat-Nr. : 80208-1-PBS

Synonyme

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

Filter:
  • Western Blot (WB) analysis of various lysates using S6 Ribosomal protein Recombinant antibody (80208-1-RR)

    WB analysis using 80208-1-RR (same clone as 80208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using S6 Ribosomal protein Recombinant antibody (80208-1-RR)

    WB analysis using 80208-1-RR (same clone as 80208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of zebrafish tissue using S6 Ribosomal protein Recombinant antibody (80208-1-RR)

    WB analysis of zebrafish using 80208-1-RR (same clone as 80208-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80208-1-RR (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using S6 Ribosomal protein Recombinant antibody (80208-1-RR)

    IF Staining of HepG2 using 80208-1-RR (same clone as 80208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using S6 Ribosomal protein antibody (80208-1-RR, Clone: 6L9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80208-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

80208-1-PBS bindet in WB, IF/ICC, Indirect ELISA S6 Ribosomal protein und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

Getestete Reaktivität human, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name ribosomal protein S6
Berechnetes Molekulargewicht 29 kDa
Beobachtetes Molekulargewicht 32 kDa
GenBank-ZugangsnummerBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.