RPS6KB2 Polyklonaler Antikörper

RPS6KB2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15268-1-PBS

Synonyme

p70 S6 kinase beta, S6K2, EC:2.7.11.1, KLS, P70 beta

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  WB analysis of MCF-7 using 15268-1-AP (same clone as 15268-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15268-1-AP (same clone as 15268-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15268-1-AP (S6K2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 15268-1-AP (same clone as 15268-1-PBS)

  IP result of anti-S6K2 (IP:15268-1-AP, 4ug; Detection:15268-1-AP 1:1000) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15268-1-AP (same clone as 15268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15268-1-AP (same clone as 15268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15268-1-AP (S6K2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15268-1-AP (same clone as 15268-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 15268-1-AP (S6K2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15268-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15268-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA RPS6KB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPS6KB2 fusion protein Ag7371
• Vollständiger Name: ribosomal protein S6 kinase, 70kDa, polypeptide 2
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC000094
• Gene symbol: RPS6KB2
• Gene ID (NCBI): 6199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS6KB2(ribosomal protein S6 kinase beta-2) is also named as 70 kDa ribosomal protein S6 kinase 2(P70S6K2),Serine/threonine-protein kinase 14B(STK14B) and belongs to the protein kinase superfamily. It is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. The activated enzyme phosphorylates several serine residues in the C terminus of ribosomal protein S6(PMID:9878560).