SAA Polyklonaler Antikörper

SAA Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 13192-1-AP

Synonyme

SAA2, Amyloid A2 protein, serum amyloid A2, Serum amyloid A-2 protein

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  WB analysis of serum from mouse injected with bacteria using 13192-1-AP

  serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 13192-1-AP (SAA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 13192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13192-1-AP (SAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 13192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13192-1-AP (SAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 13192-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SAA antibody (13192-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Bakterien injiziertes Serum aus der Maus
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

13192-1-AP bindet in WB, IHC, IF/ICC, ELISA SAA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAA fusion protein Ag3869
• Vollständiger Name: serum amyloid A2
• Berechnetes Molekulargewicht: 122 aa, 14 kDa
• Beobachtetes Molekulargewicht: 12-13 kDa
• GenBank-Zugangsnummer: BC020795
• Gene symbol: SAA2
• Gene ID (NCBI): 6289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human SAA proteins are a group of apolipoproteins mainly found in the high density lipoprotein (HDL) portion of plasma. The serum amyloid A (SAA) protein is an acute phase apolipoprotein reactant produced mainly by hepatocytes and under the regulation of inflammatory cytokines(PMID: 12011456). Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA2 protein(PMID: 1463770). This antibody can recognize SAA1 and SAA2.

Publikationen

Species	Application	Title
mouse	IHC	J Crohns Colitis; Lysophosphatidylcholine-Induced Aberrant Adipogenesis in Mesenchymal Stem Cells and Impaired Antibacterial Function in Adipocytes of Creeping Fat; Authors - Fangting Wu
human	WB,IF	Inflammation; Interleukin-1β Induces Intracellular Serum Amyloid A1 Expression in Human Coronary Artery Endothelial Cells and Promotes its Intercellular Exchange.; Authors - Tadeja Kuret
mouse	WB	Int Immunopharmacol; Artesunate attenuates serum amyloid A-induced M1 macrophage differentiation through the promotion of PHGDH; Authors - Xinhui Lu
human	IHC	Oncol Lett; Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma; Authors - Wu-Fu Chen
mouse	IF	bioRxiv; Single cell RNA sequencing reveals a shift in cell function and maturation of endogenous and infiltrating cell types in response to acute intervertebral disc injury; Authors - Sade W Clayton