SAC3D1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SAC3D1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 25857-1-AP

Synonyme

SHD1, SAC3 domain containing 1

---

• 

  WB analysis using 25857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25857-1-AP (SAC3D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25857-1-AP (SAC3D1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 25857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25857-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25857-1-AP (SAC3D1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

25857-1-AP bindet in WB, IHC, IF, ELISA SAC3D1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAC3D1 fusion protein Ag23026
• Vollständiger Name: SAC3 domain containing 1
• Berechnetes Molekulargewicht: 404 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44, 31 kDa
• GenBank-Zugangsnummer: BC007448
• Gene symbol: SAC3D1
• Gene ID (NCBI): 29901
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAC3D1, also named as SHD1 and HSU79266. SAC3D1 is widely found in the cytoplasm, cytoskeleton, microtubule, tissue center, centrosome and spindle, involved in centrosome duplication and mitotic progression. SAC3D1 is abnormally expressed in multiple types of cancer, such as colon cancer and hepatocellular carcinoma and gastric cancer. SAC3D1 may serve as a prognostic biomarker in hepatocellular carcinoma (PMID: 30353105, 32319600). SAC3D1 has 2 isoforms with the molecular mass of 44 and 31 kDa.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Front Genet; Conduction and validation of a novel mitotic spindle assembly related signature in hepatocellular carcinoma: prognostic prediction, tumor immune microenvironment and drug susceptibility; Authors - Zhao Zhang ; KD Validated
Human	WB,IHC	Mol Med Rep; SAC3D1 activates Wnt/β‑catenin signalling in hepatocellular carcinoma; Authors - Haitao Wang