SAFB Polyklonaler Antikörper

SAFB Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (6)

Kat-Nr. : 21857-1-AP

Synonyme

SAF B1, SAF B, HSP27 estrogen response element-TATA box-binding protein, HSP27 ERE-TATA-binding protein, HET

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  WB analysis of MCF-7 using 21857-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21857-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21857-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21857-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21857-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21857-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21857-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21857-1-AP (SAFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.

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  IHC staining of mouse testis using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21857-1-AP (SAFB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.

• 

  IHC staining of mouse testis using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21857-1-AP (SAFB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 21857-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 21857-1-AP (SAFB Antibody) at dilution of 1:50.

• 

  IF Staining of A431 using 21857-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SAFB antibody (21857-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, COLO 320-Zellen, HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Mammakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 4 publications below

Produktinformation

21857-1-AP bindet in WB, IHC, IF/ICC, ELISA SAFB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAFB fusion protein Ag16451
• Vollständiger Name: scaffold attachment factor B
• Berechnetes Molekulargewicht: 917 aa, 103 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC126219
• Gene symbol: SAFB
• Gene ID (NCBI): 6294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport. hnRNPs also bind heterogeneous nuclear RNA (hnRNA), the transcripts produced by RNA polymerase II. SAFB (scaffold attachment factor B) is a nuclear matrix-associated protein that binds to matrix- or scaffold-associating regions (MARs or SARs) on DNA and interacts with RNA polymerase II and serine-/arginine-rich RNA processing factors (SR proteins). SAFB, also designated HAP (hnRNP A1 associated protein) and HET (HSP 27-ERE-TATA-binding protein) is a proven hnRNP protein that has a speckled distribution in the nucleus and, in response to stress agents such as heat shock, is recruited to a few, large nuclear granules, called perichromatin granules. SAFB also binds to the estrogen receptor (ER) and is expressed in several breast cancer cell lines at varying levels. Subsequently, SAFB may play a role in breast cancer by mediating cellular proliferation and division. This antibody specifically reacts whith the isoform 1 of human SAFB protein. SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SAFB is 103 kDa, but the modified SAFB is about 130-175 kDa.(PMID: 28469965, PMID: 26273616 )

Publikationen

Species	Application	Title
human	IF	Acta Neuropathol Commun; RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.; Authors - Mahlon Collins
human	IF	Neurobiol Dis; ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity.; Authors - Haiyan An
mouse	IF	Mol Cell; SAFB restricts contact domain boundaries associated with L1 chimeric transcription; Authors - Yaqiang Hong
human	IF	Cell Rep; Stress-induced TDP-43 nuclear condensation causes splicing loss of function and STMN2 depletion; Authors - Wan-Ping Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (12-16-2024)

Antibody does recognise the band of the expected molecular weight of SafB protein, however other non-specific bands are also visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RKO

SAFB Antibody Western Blot validation (1:1000 dilution) in RKO (Cat no:21857-1-AP)

FH

Tsimafei (Verified Customer) (11-19-2024)

Antibody detect a band below 150 kDa in HEK293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

SAFB Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:21857-1-AP)

FH

Izortze (Verified Customer) (10-02-2024)

Great antibody for immunoprecipitation!

• Applications: Immunoprecipitation
• Cell Tissue Type: EndoCb-H1 cell line

SAFB Antibody Immunoprecipitation validation ( dilution) in EndoCb-H1 cell line (Cat no:21857-1-AP)

FH

Julia (Verified Customer) (08-07-2024)

Used antibody for mESC protein extract. Band of the correct size is visible, additional band at ~75kDa. Stains ladder but otherwise good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESC

SAFB Antibody Western Blot validation (1:5000 dilution) in mESC (Cat no:21857-1-AP)

FH

Tatyana (Verified Customer) (12-04-2023)

Good signal in ICC, can be used to detect nuclear stress bodies (nSBs)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells

SAFB Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:21857-1-AP)

FH

Bibha (Verified Customer) (06-05-2019)

I used this antibody in human primary astrocytes Mock-infected or infected with Venezuelan equine encephalitis virus.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary astrocytes

SAFB Antibody Western Blot, validation (1:1000 dilution) in Human primary astrocytes (Cat no:21857-1-AP)