SAFB2 Polyklonaler Antikörper

SAFB2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (3)

Kat-Nr. : 11642-1-AP

Synonyme

KIAA0138, SAF B2, SAF-B2

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  WB analysis of MCF-7 using 11642-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 11642-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 11642-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11642-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11642-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11642-1-AP (SAFB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11642-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11642-1-AP (SAFB2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11642-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11642-1-AP (SAFB2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 11642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAFB2 antibody (11642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, BxPC-3-Zellen, HeLa-Zellen, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

11642-1-AP bindet in WB, IHC, IF/ICC, ELISA SAFB2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAFB2 fusion protein Ag2230
• Vollständiger Name: scaffold attachment factor B2
• Berechnetes Molekulargewicht: 528aa,57 kDa; 953aa,107 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC025279
• Gene symbol: SAFB2
• Gene ID (NCBI): 9667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

Publikationen

Species	Application	Title
mouse	WB,IF	Proteomics; Establishment of a Proteomic Profile Associated with Gonocyte and Spermatogonial Stem Cell Maturation and Differentiation in Neonatal Mice.; Authors - Bo Zheng
mouse	WB	RNA; SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells; Authors - Rachel E Cherney

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (08-30-2025)

Very good and specific antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: U2OS

SAFB2 Antibody Western Blot validation (1:8000 dilution) in U2OS (Cat no:11642-1-AP)

FH

Aleksandr (Verified Customer) (07-29-2025)

Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: RKO

FH

Julia (Verified Customer) (12-05-2024)

Good antibody, seems to be recognising another protein at ~130kDa likely Safb.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESC

SAFB2 Antibody Western Blot validation (1:10000 dilution) in mESC (Cat no:11642-1-AP)