SAFB2 Polyklonaler Antikörper

SAFB2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11642-1-PBS

Synonyme

KIAA0138, SAF B2, SAF-B2

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  WB analysis of MCF-7 using 11642-1-AP (same clone as 11642-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

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  WB analysis of rat pancreas using 11642-1-AP (same clone as 11642-1-PBS)

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11642-1-AP (same clone as 11642-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 11642-1-AP (same clone as 11642-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 11642-1-AP (same clone as 11642-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11642-1-AP (same clone as 11642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11642-1-AP (SAFB2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11642-1-AP (same clone as 11642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11642-1-AP (SAFB2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 11642-1-AP (same clone as 11642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11642-1-AP (SAFB2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 11642-1-AP (same clone as 11642-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAFB2 antibody (11642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11642-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11642-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SAFB2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAFB2 fusion protein Ag2230
• Vollständiger Name: scaffold attachment factor B2
• Berechnetes Molekulargewicht: 528aa,57 kDa; 953aa,107 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC025279
• Gene symbol: SAFB2
• Gene ID (NCBI): 9667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.