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SAGE1 Polyklonaler Antikörper

SAGE1 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30914-1-PBS

Synonyme

sarcoma antigen 1, SAGE, CT14, Cancer/testis antigen 14

Filter:
  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SAGE1 Polyclonal antibody (30914-1-AP)

    IHC staining of human skin cancer using 30914-1-AP (same clone as 30914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using SAGE1 Polyclonal antibody (30914-1-AP)

    IHC staining of rat testis using 30914-1-AP (same clone as 30914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SAGE1 Polyclonal antibody (30914-1-AP)

    IHC staining of mouse testis using 30914-1-AP (same clone as 30914-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30914-1-AP (SAGE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using SAGE1 Polyclonal antibody (30914-1-AP)

    IF Staining of A375 using 30914-1-AP (same clone as 30914-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SAGE1 Polyclonal antibody (30914-1-AP)

    IF Staining of U2OS using 30914-1-AP (same clone as 30914-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAGE1 antibody (30914-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30914-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30914-1-PBS bindet in IHC, IF/ICC, Indirect ELISA SAGE1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SAGE1 fusion protein Ag34216
Vollständiger Name sarcoma antigen 1
Berechnetes Molekulargewicht99kd
GenBank-ZugangsnummerNM_018666.2
Gene symbol SAGE1
Gene ID (NCBI) 55511
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).