SAGE1 Rekombinanter Antikörper

SAGE1 Rekombinant Antikörper für IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230094C11

Kat-Nr. : 82876-1-PBS

Synonyme

230094C11, Cancer/testis antigen 14, CT14, SAGE, sarcoma antigen 1

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  IF Staining of MDA-MB-453 using 82876-1-RR (same clone as 82876-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDA-MB-453 cells using SAGE1 antibody (82876-1-RR, Clone: 230094C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82876-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82876-1-RR (same clone as 82876-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SAGE1 (82876-1-RR, Clone:230094C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82876-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82876-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82876-1-RR against Human SAGE1 were performed. The affinity constant is 0.546 nM.

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Geprüfte Anwendungen

Produktinformation

82876-1-PBS bindet in IF/ICC, FC (Intra), Indirect ELISA SAGE1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SAGE1 fusion protein Ag34216
• Vollständiger Name: sarcoma antigen 1
• Berechnetes Molekulargewicht: 99kd
• GenBank-Zugangsnummer: NM_018666.2
• Gene symbol: SAGE1
• Gene ID (NCBI): 55511
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596).