SALL4 Polyklonaler Antikörper

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• KD/KO Validated

SALL4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 24500-1-AP

Synonyme

Zinc finger protein SALL4, Zinc finger protein 797, Sal-like protein 4, Sal like protein 4, HSAL4

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  WB analysis of HepG2 using 24500-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24500-1-AP (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24500-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24500-1-AP (SALL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 24500-1-AP

  IP result of anti-SALL4 (IP:24500-1-AP, 4ug; Detection:24500-1-AP 1:600) with HepG2 cells lysate 3600ug.

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  IHC staining of human testis using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:50.

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  IHC staining of mouse testis using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24500-1-AP (SALL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:50.

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  IHC staining of human ovary tumor using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of Caco-2 using 24500-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (24500-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Ovarialkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

24500-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA SALL4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SALL4 fusion protein Ag17480
• Vollständiger Name: sal-like 4 (Drosophila)
• Berechnetes Molekulargewicht: 1053 aa, 112 kDa
• Beobachtetes Molekulargewicht: 66-75 kDa
• GenBank-Zugangsnummer: BC111714
• Gene symbol: SALL4
• Gene ID (NCBI): 57167
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sal-like protein 4 (SALL4), also named Zinc finger protein 797, contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is a transcription factor that exists in two splice isoforms and regulates transcription in embryonic stem cells, hematopoiesis, and acute myeloid leukemia. SALL4 is activated in fetal liver tissue, silenced in adult liver tissue, and reexpressed as an oncofetal protein in hepatocellular carcinoma (PMID: 26892498, 23758232). SALL4 has two isoforms with predicted molecular weights of 112 kDa (SALL4A) and 66 kDa (unmodified SALL4B), and SALL4B was found to be the dominant endogenous isoform in HCC cell lines. Ectopically expressed SALL4B migrated at 95 kDa modified by ubiquitination, 130 kDa modified by sumoylation (PMID: 23012367).

Publikationen

Species	Application	Title
human	IF	Sci Rep; Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.; Authors - Honghai Xia
human	IHC,WB	Cell Signal; miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.; Authors - Dong-Min Shi
human	WB	Onco Targets Ther; PMID: 31632072; Authors - Chiung-Chi Cheng
human	WB	Sci Rep; MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1.; Authors - Yancheng Lin
human	WB	Pathol Int; Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1; Authors - Qing-Dong Wang
human	WB,IHC,IF	J Mol Histol; Expression and clinical significance of SALL4 and β-catenin in colorectal cancer.; Authors - Liliang Hao