SAMD4B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SAMD4B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17723-1-AP

Synonyme

hSmaug2, Protein Smaug homolog 2, SAM domain-containing protein 4B, Smaug 2, SMAUG2

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  WB analysis of HeLa using 17723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17723-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17723-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17723-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 17723-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17723-1-AP

  Immunofluorescent analysis of HepG2 cells, using SAMD4B antibody 17723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Mausnierengewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, Mausnierengewebe, Maus-Pankreasgewebe, Rattennierengewebe, Ratten-Pankreasgewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

17723-1-AP bindet in WB, IHC, IF/ICC, ELISA SAMD4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAMD4B fusion protein Ag12113
• Vollständiger Name: sterile alpha motif domain containing 4B
• Berechnetes Molekulargewicht: 694 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC054518
• Gene symbol: SAMD4B
• Gene ID (NCBI): 55095
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sterile alpha motif domain-containing protein 4B (SAMD4B) is also named as Smaug2. SAMD4B is a mammalian homologue of the Smaug protein of Drosophila melanogaster and can participate in the process of regulating RNA stability as a posttranscriptional inhibitor (PMID: 23592795). SAMD4B is expressed in most human embryos and adult tissue (PMID: 23592795). SAMD4B, especially its expression level, could affect the immune microenvironment (PMID: 38886351).

Publikationen

Species	Application	Title
human	WB	Cell Mol Immunol; SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.; Authors - Yuze Wang ; KO Validated
human	WB,IF	Mol Med Rep; miR‑451 suppresses the malignant characteristics of colorectal cancer via targeting SAMD4B.; Authors - Chunrong Wu